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54, 55 Specifically, plasma samples were deproteinated by adding 50% v/v of freshly prepared 10% metaphosphoric acid (MPA), centrifuging at 2000 g for 2 min and then the supernatant stored at 20 C until assaying

Evidence of increased immune cell activation and increased free radical production in the areas of the brain that are selectively damaged suggests that oxidative stress plays an important role in the neurologic pathology of thiamin deficiency (13)

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Mice received 200 L subcutaneous saline and were left to recover on a heating pad for 30 minutes with regular chow and moistened fines added to the cage