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Apoptotic bodies derived from fibroblast-like cells in subcutaneous connective tissue inhibit ferroptosis in ischaemic flaps via the mir-339-5p/KEAP1/nrf2 axis

Briefly, the slides were incubated with 3% H 2 O 2 to eliminate endogenous peroxidase activity and then blocked with 5% BSA followed first by incubation with primary antibodies against VEGFA (1:200) and anti-IL8 (1:200) overnight at 4 C, and second by incubation with a secondary antibody

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