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Waters, Eschborn, Germany) at 50 C with a flow rate of 0.3 mL/min and a mobile phase consisting of methanol-water-acetic acid of 42:58:0.01 (v/v/v) that was ramped to 86:14:0.01 (v/v/v) over 12.5 min and then to 98:2:0.01 (v/v/v) for 3 min 114

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The QC samples were used to monitor the stability and functionality of the system throughout the sample analysis (3 QCs per sample type)

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Diabetologia2007 Jan;50(1):168-77