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They are all small molecules which act as reversible competitive inhibitors of the catalytic activity of DPP-4 and, when used at the appropriate therapeutic dose, are associated with at least 70% inhibition of plasma DPP-4 activity ( in vivo is likely to be higher, due to dilution of the inhibitor in the ex vivo assay)

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semaglutide Generally, the more expensive of the two

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The assay is optimized to operate at an acidic pH of 5.0, which enhances the stability of the colored complex and minimizes interference from competing reactions