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12 m cryosections were analyzed for hematoxilin/eosin (H&E), immunofluorescence, immunohistochemistry and picrosirius red staining (BioOptica) according standard procedures

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While a substitution with the polar serine at position 13 retained wild-type dehydratase activity, presumably due to the alcohols ability to assume cysteines function in stabilizing deprotonated GS , the only other Pt GSTF8 variants to keep analbeit reducedflavan-3,3,4-triol ( 4 ) dehydration activity contained a glycine, alanine or asparagine mutation (Fig