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In: Shils ME, Olson JA, Shike M, Ross AC, eds

After centrifugation at 1000 x g for 10 min, the supernatant (S1) was centrifuged for 20 min at 17.500 x g to yield the supernatant S2 and the pellet P2 (crude synaptosomal fraction)

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and Walling, C., 1993