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The increase of absorbance at 405 nm in samples was measured using a Tecan Infinite M1000 or Tecan Spark multiplate readers (Tecan AG) for 30 min at 37 C

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Whole-body composition was measured in unanesthetized rats before and after surgery using an EchoMRI 100 whole-body composition analyzer (EchoMRI, Houston, TX, USA)

p=0.392), and other steroids (sHR=1.045, p=0.928) did not significantly predict different 30-day mortality (Figure)

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