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Additionally, we analyzed shortest paths for the AMPK signaling pathway (figure not shown) where the top three targets were G6PC, PPARG and PPARGC1A

DIA MS2 scans were acquired in the Astral mass analyser with a 3 ms maximum injection time using a variable windowing scheme, using 2 Da windows from 380 to 680 m/z , 4 Da windows from 680 to 800 m/z and 8 Da windows from 800 to 980 m/z

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NCOA4-silent KGN was finally obtained by examining mRNA after 2472 h of transfection and protein levels after 4896 h at 37 C