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Microstructure and volume No substantial differences emerged in the microstructure of white matter (WM) pathways (mean FA difference [95%-CI]: 0.001 [ 0.0140.011], P = 0.815), gray matter (GM) in the target region (mean MD difference [95%-CI]: 5.3e 05 [ 1.2e 042.3e 05], P = 0.163), or volumes of WM tracts (mean difference [95%-CI]: 15.6 [ 397.5366.2], P = 0.933) and GM targets (mean difference [95%-CI] 1.1e 04 [ 1.9e 044.2e 04], P = 0.460) after the intervention between the anodal and sham stimulation groups (see Additional file 1: Table S2)

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Luciferase activity was quantified using a dual-luciferase assay system (Promega, E1910)

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