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What warrants attention and a call to the clinic: persistent nausea beyond the first 10 days, injection site reactions that worsen rather than resolve, or any signs of systemic allergic response

During this reaction, GSH is oxidized into GSSG

The tips were washed twice with 200 l 0.1% FA, followed by elution of the peptides with 100 l 40% acetonitrile/0.1% FA by centrifuging for 4 min at 1,500 g