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Long-term effects of stimulant medications on the brain: possible relevance to the treatment of attention deficit hyperactivity disorder

The membranes were then incubated with the ER (Abcam, Cambridge, HK), CYP3A4 (Abcam, Cambridge, HK) and GAPDH (Proteintech, wuhan, China) overnight at 4 C, followed by washing with TBST 10 min for 3 times

B12 helps release energy from food, so a person whose blood levels are genuinely low can feel a real lift once treated

The related proteins were detected by Western blot

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