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Secreted insulin was analysed from a sample of diluted supernatant by immunoassay (Cisbio wide range HTRF assay, measured using a Spectramax i3x plate reader)

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The basal insulin dose was decreased by 20% at randomization and liraglutide injection was titrated weekly by 0.6 mg for 2 to 3 weeks based on tolerability and an average fasting plasma glucose goal of 110 mg/dL