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For the non-targeted metabolite analysis, the tissue metabolites were extracted in 80% MeOH at a final tissue concentration of 50 mg/mL for 24 h at 80 C

Yes, body weight is a significant factor

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Sources Food sources Cruciferous vegetables Cruciferous vegetables, such as bok choi, broccoli, Brussels sprouts, cabbage, cauliflower, horseradish, kale, kohlrabi, mustard, radish, rutabaga, turnip, and watercress, are rich sources of glucosinolate precursors of isothiocyanates (63)