Also, the plasma levels of soluble intercellular adhesion molecule-1 (ICAM-1) and CD4 + T-cells expressing C-C chemokine receptor 6 (CCR6) are reduced in EAE mice treated with EGCG (Wang et al., 2012
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For method 3, magnetically enriched cultures at 90% parasitaemia and 0.12% Hct were incubated with the test compounds for the desired times (drug treatments, 100 nM DHA for 3 h, 300 nM OZ277 for 3 h, 300 nM OZ439 for 6 h, 1 M pyrimethamine for 5 h, and three control conditions, 1 mM DIAM for 5 min, 10 mM DTT for 5 min and DMSO for 6 h)
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