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In vitro experiments further revealed that under high glucose conditions, marked increase in the expression of TRIM32 along with a concomitant downregulation in the AKT and mTOR phosphorylation levels was observed, which further exacerbated pancreatic cell autophagy and hampered insulin secretion, thereby promoting development of type 2 diabetes

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After extraction at 20 C and 180 rpm for 2 h on a shaker, the upper organic layer containing fatty acids was collected and esterified with methanol to generate fatty acid methyl esters (Khosa et al

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