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An EM GP cryopreparation system (Leica Microsystems, Buffalo Grove, IL) was used for plunge-freezing with the following parameters: 5 s pre-blot time, 2.05.0 s blotting time, followed by 0.5 s post-blot time

DMEM high glucose medium and ELISA kits were purchased from Keygen Biotech Corp., Ltd

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instead of uterine contractions, milk is ejected from the breast

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Mental focus: Acetylcholine supports attention, concentration, and reaction speed during training