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DNMT1 mediates metabolic reprogramming induced by Epstein-Barr virus latent membrane protein 1 and reversed by grifolin in nasopharyngeal carcinoma

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Western blot analysis The kidney organoids were homogenized in boiling lysis buffer (1% SDS, 1 mM sodium orthovanadate, and 10 mM Tris, pH 7.4), and the protein concentration was determined with a BCA Protein Assay Kit (Pierce Biotechnology Inc., Rockford, IL, USA)

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5.1.6 Anti-fatigue effect Polysaccharides from PCH exhibit anti-fatigue activity by preventing excessive accumulation of metabolites, reducing muscle damage, delaying oxidative injury, and modulating gut microflora ( The mechanism for this anti-fatigue was related to enhancing osteocalcin-mediated interaction between muscles and bones

3.3 Multiple sclerosis Multiple sclerosis (MS) is an inflammation-mediated demyelinating disease of the human central nervous system, with neurodegeneration being the primary cause of irreversible neurological disability