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For samples obtained from Leipzig University Hospital, the perfusion procedure differed slightly: solution A (composed of 10 mM HEPES (Carl Roth), 143 mM NaCl, 6.7 mM KCl, 2.4 mM EGTA, 5 mM N -acetyl-l-cysteine, 11 mM d-glucose (all provided by Sigma-Aldrich) and 32 U l 1 human insulin (Eli Lilly) in double-distilled water (pH 7.4)) at 39 C with a rate of 25 ml per minute for at least 20 min

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Environmental and clothing adjustments: Layered clothing : Wearing multiple thin layers traps air and provides better insulation than single heavy garments

A: Skin: 4-8 weeks

Retrieved October 6, 2015