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As we grow older, our cells produce less glutathione, slowing down the body's antioxidant defences

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These metabolites can be broadly classified into water-soluble antioxidantssuch as proline, glycine betaine, ascorbic acid (AsA), and glutathione (GSH)and lipophilic antioxidants, including tocopherols and carotenoids, reflecting both their chemical properties and intracellular compartmentalization (Mishra et al

Briefly, urine metabolites were purified using Discovery C18 SPE cartridges (SPE 96 well plate, 100 mg/well, 2 ml)