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A total of 10 l of CCK-8 solution was added to each well, and the cells were incubated for 1 h in the incubator

Within the cardiovascular system, L-citrulline enhances NO bioavailability by promoting the phosphorylation and activation of endothelial NO synthase (eNOS), thereby improving endothelial function ( Figure 2b ) ( Figure 2c ) ( L-citrullines regulatory influence further extends to immune and metabolic systems

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