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Initially, the enzyme -glutamyl cysteine synthetase (-GCS) facilitates the combination of cysteine with glutamate, leading to the creation of -glutamylcysteine

It is important to note that there are currently some negative results of ASK1-related small molecule inhibitors (such as CFLAR-mimicking peptide) in clinical trials

It plays a fundamental role in numerous metabolic and biochemical reactions such as DNA synthesis and repair, protein synthesis, prostaglandin synthesis, amino acid transport, and enzyme activation

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Khan et al., 2017

Targeted LC-MS/MS is suitable for focused hypotheses requiring rigorous stabilization and quantification, whereas discovery-oriented studies may combine broader profiling with targeted follow-up