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These simple precautions only take a few moments but contribute to safer treatment

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For assessing the change in relative IBA1 or GFAP abundance at different doses of LPS, a linear model was constructed for each protein (IBA1 or GFAP) and each brain region (hippocampus or entire brain) using the protein percentage as dependent variable and treatment (veh-PBS, veh-LPS-0.05, veh-LPS-0.1, veh-LPS-0.5, and veh-LPS-1.0) and time (2d and 11d after the last LPS injection) as independent variables with interactions effects

Dosage: The dosage begins at 0.25 mg and is gradually increased over several weeks by a medical professional, based on requirements and tolerance

The plates were incubated overnight at 37 o C in an atmosphere of 95% O 2 and 5% CO 2

Statistical analysis Data are presented as means SD