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In experimental models for the induction of tissue damage, 20 mg/kg MTX was a commonly used dose for rats (Reference Moodi, Hosseini and Abedini16Reference Azadnasab, Kalantar and Khorsandi20)

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The millimolar GSH intracellular concentrations, the low plasma micromolar concentrations and the low GSH redox potential ( E 0 = 240 mV) make GSH an ideal and perfect cellular redox buffer (1618)

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The fresh tissue was thoroughly homogenised in liquid nitrogen following extraction in ice-cold buffer containing100 mM TrisCl (pH 7.8) buffer containing 10 mM MgCl 2 , 1 mM PMSF, 10 mM DTT, 100 mM EDTA, and 2% PVP, 0.1% protease inhibitor

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