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ES2, OVCAR3 and OVCAR8 cell lines were cultured in DMEM (41965039, Gibco, Life Technologies) supplemented with 1% FBS (S 0615, Merck), 1% antibiotic-antimycotic (AA) (P0607300, PAN Biotech)

The immune system either reacts or it does not, and this is usually apparent quickly

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Acknowledgements The authors would like to acknowledge the Instituto Portugus de Oncologia de Lisboa Francisco Gentil (IPOLFG) for partially funding the project

This may be due to B12s role in DNA synthesis and red blood cell production when disrupted, it affects the supply of nutrients to hair follicles