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The chimeric reporter protein of FIXgla-Fas with a HPC4 tag (EDQVDPRLIDGK) and a flexible linker GGSGG following FIXgla was obtained by overlap PCR and cloned into pcDNA3.1 vector

Izano EA, Amarante MA, Kher WB, Kaplan JB

Daily injections over weeks allow cumulative peptide buildup in retinal tissues explaining delayed onset of benefits (several weeks typical in studies)