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Secretome sample preparation For secretome analysis, 5 mL of conditioned medium was collected from both treated and control cell cultures

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Puri P, Baillie RA, Wiest MM, Mirshahi F, Choudhury J, Cheung O, Sargeant C, Contos MJ, Sanyal AJ (2007) A lipidomic analysis of nonalcoholic fatty liver disease

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HPLC ensures that the peptide is at least 99% pure, stripping away any toxic residual solvents leftover from manufacturing

Visible improvement typically requires 48 weeks