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doi: 10.3390/cancers12020448 74 Preston-MartinSFranceschiSRonENegriE

Blood was first diluted 1:1 in PBS, and PBMCs were isolated using SepMate-50 separation tubes (STEMCELL Technologies) according to the manufacturers instructions

To corroborate our IHC data, western blotting experiments for TNF- and IL-1 showed that their expressions were significantly increased after FA injection, which were alleviated by LA supplementation but without significant difference between the two dose groups, shown in Figure 3

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