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For enrollment, subjects had to demonstrate stable facial hyperpigmentation for at least 3 months, macular lesions that were neither depressed nor atrophic, and melasma severity scores of 2 or higher

152, 153 In humans, circulating NO concentration increases after the consumption of polyphenols

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However, for concentrations exceeding three g/dl, dilution with a 0.1 M phosphate buffer at pH 7.0 was necessary to obtain reliable results

Springer Nature Singapore, pp 181214