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Misaligned expectations are the most common reason patients discontinue prematurely

This approach helps preserve comparability across runs and allows later replications to be performed under comparable assumptions

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By splitting an aptamer that binds to ATP and labeling each fragment with differently colored fluorophores, they were able to develop an assay that reports binding only when the two fluorophores are in proximity to each other, and thus eliminates background from non-specific binding events

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