These investigations contribute to a broader understanding of peptide biology and how short amino-acid sequences may influence cellular processes within experimental environments
Among the former, fluorescein staining of the ocular surface was scored according to the recommendations of the National Eye Institute/Industry workshop on clinical trials for dry eyes (NEI-SS) ( Tear samples were collected at visits V1, V3, V4, and V6 using Schirmer strips, as previously described in the literature ( 2.6 Analysis of cytokines in tears and HCEpiC cell cultures To determine cytokine levels from recovered tears and supernatant from treated HCEpiC cells, the LEGENDplex Human Inflammation Panel 1 (13-plex) flow cytometry-based assay kit was used (BioLegend, San Diego, CA)
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To eliminate formazan crystals, 200 Lof DMSO was then replaced with the media, and absorbance values were measured at 570 nm, using a spectrophotometric Plate Reader (ELx808, Lonza Biotech Co., Switzerland)
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