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The scientists reported this work in a paper published in Advanced Healthcare Materials .

Early studies of hepatic-portal glucagon infusions in rodents reduced the size of spontaneous meal intake without affecting intermeal intervals, indicating a contribution to meal termination rather than postprandial satiety [79, 80]

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Oocytes were first fixed in 4% paraformaldehyde and permeabilized using Triton X-100, followed by blocking in 1% BSA