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Through the recognition of unique antigens (e.g., tumor-specific antigens) present on target cells or tissues, ADCs can deliver drugs with potent cytotoxic or immunomodulatory activity, offering favorable bioavailability and relatively lower adverse reactions [2]

Then, electrophoresis was performed at a constant voltage of 150 V using a mini-gel slab device Mini Protean III (Bio-Rad, Hercules, CA)

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Vitamin C is also a potent anti oxidant, that along with GSH compete for removal of free radicals in the cell

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Copper content determination Copper content was determined for jr PPO1-wt and the five investigated mutants spectrophotometrically by measuring the absorption (546 nm, = 6300 M 1 cm 1 ) of a Cu I -2, 2-biquinoline complex