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The rats were deeply anesthetized intraperitoneally using thiopental and diazepam

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Common research applications include receptor binding assays, cell culture studies, structure-activity relationship analysis, and bioassay development

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Autonomic nervous system activity changes, particularly alterations in sympathetic and parasympathetic tone, can modulate intestinal blood flow, mucus secretion, and immune cell function, indirectly influencing the microbial ecosystem ( 2.1.3 Key signaling pathways Signal transmission within the MGBA involves multiple key pathways, including neural, immune, endocrine, and metabolic pathways, which interact to form a complex regulatory network