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This is why a minor stressor can trigger a massive symptom flare-up years later [6]

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RNA extraction and RTqPCR Total RNA from cells was isolated via TRIzol (Takara, Otsu, Japan) and subsequently purified with chloroform/isopropanol/ethanol, and cDNA was synthesized via the PrimeScript RT reagent Kit (Takara) according to the manufacturers instructions and used as the template for qRTPCR

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@ludilin discusses with @ebenezersamuel23 and @dr.patdavidson when he joins #strongtalk

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