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For ELISA, liver tissues were sonicated in 50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% Triton X-100 and protease inhibitor cocktail (EDTA-free Complete Mini, Roche)

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Also, while the follow-up time was substantial (averaging 2.7 years for GLP-1 users), longer-term effects couldnt be assessed

They'll argue that since Ozempic isn't a designated weight loss drug, they are not obligated to cover it for that purpose

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This can leave your cells more vulnerable to oxidative stress and slow down your ability to recover