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At the time of assay, the growth medium was replaced with HBSS, supplemented with 10 mM HEPES and 0.1% (w/v) ovalbumin and incubated at room temperature for 30 min

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Due to changes in data collection practices over time and staggered onboarding during the study period, the number of participants reporting weight data decreased at subsequent time points, comprehensive demographic and clinical characteristics were available for a subset of 1864 participants who enrolled at the inception of the service

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