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The tumor tissues were incubated overnight at 4C in the following IHC stains, then incubated in secondary antibodies: P-gp (1:500)
Orthotopic xenograft tumour models were performed at external contract research organisations under funding from Jumpstart Fertility and Metro Biotech
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However, in the normal physiological conditions follicular fluid NO seems to be synthesized from granulosa cells by endothelial NO synthase, since in isolated human follicular cells at least 90% of cells are granulosa cells even though macrophages and lymphocytes are present as well
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