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A 6-week study conducted to assess soil acidity effects on earthworms showed that low pH (3.4) and high Al levels (1000 mg Al kg 1 soil) inhibited cocoon production and caused all earthworms to die [380, 381]

[DOI] [Google Scholar] 225.Mahady G.B

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Since AAVs have been found to integrate within DSBs with high frequency [152], the coupling of CRISPR-Cas9 with AAVs as the delivery vector must be carefully evaluated before clinical translation [58, 153]