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However, by immunoneutralization in rats, it turned out to be impossible to completely remove the incretin effect (24), and experiments in which the incretin effect was compared with the GIP responses to glucose in people with various resections of the small intestine, confirmed that the incretin effect was not correlated with the GIP responses and upper small intestinal conservation, but rather with the preservation of distal small intestinal segments (25)
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So how can we avoid these problems
mouse anti-human Tom20 (F-10) (Santa Cruz Biotechnology, sc-17764, 1:1000)
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