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Did travel, stress, or sleep disruption affect routine

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For each compound five independent biological replicates were performed

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However, it is essential to understand that results can vary from person to person

Given that MTX is more effective against dividing cells because of its effect on the nucleotide synthesis, we also examined correlations between metabolites concentrations and cell proliferation rate, as this could be a confounding factor (proliferation curves for doubling time determination are in Supplemental Figure 5 )