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Plates were incubated at room temperature for 30-60 min in the dark, and fluorescence was read at Ex/Em = 490/520 nm

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The reason why people are interested in the technique is cosmetic, he says of his patients, but they are cosmetic based on family issues, psychological problems, social problems. His first patients were brides whose wedding nights were approaching, and whose husbands hadnt seen their eyes without color contacts

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