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Motif analysis using the MEME 42 revealed a notable enrichment of DAF-16 binding motif among the genes differentially expressed in glp-1(-) and fem-3(-) worms

This finding has direct implications for research protocol design: most published and structured study designs incorporate a rest period following the active phase to allow receptor sensitivity to normalize before any subsequent research cycle, rather than using continuous, uninterrupted administration

Given the therapeutic potential of both dietary interventions as well as GLP-1 analogs, understanding how dietary interventions affect circulating GLP-1 can help further improve IBS treatment

52 In addition, Lei et al