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The system was set to a flow rate of 0.060 mL/min and composed of 5 mM ammonium formate in a gradient of water/acetonitrile concentrations (all mobile phase solvents were Optima LC/MS Grade, and purchased from Fisher Scientific, Fair Lawn, NJ)

The role of Zn dyshomeostasis in obesity is also confirmed by the results of supplementation trials

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Liraglutide (GLP-1) Liraglutide is a GLP-1 receptor agonist with 97% amino acid sequence homology to native GLP-1, modified with a palmitic acid chain for extended duration of action, used in research for diabetes and obesity.