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It has been demonstrated that eryptosis might alter cell volume, shape, granularity of erythrocytes, as well as modify the phospholipid composition of both inner and outer leaflets of phospholipid bilayers in the cell membrane, leading primarily to PS externalization [85, 86]

At 18 h after transfection, medium was replaced and cells were cultured for an additional 30 h prior to incubations

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