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Mutation of RA2 domain of PLC only partially inhibited this stimulation suggesting that GPCR can stimulate PLC in RA2-dependent and RA2-independent way [11]

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Following 24 h of incubation, the culture medium was replaced with 500 L medium with/without serum (depends on experiment, as a controls) or 500 L of the appropriate medium containing various concentration of DMSO (1%, 2%, 3%, 4%, 5% and 10%), and then the cells were cultured for 24 h

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So, buckle up for a straightforward exploration of IGF-1 LR3