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After 48 h, biofilms were washed once with 1 PBS followed by treatment with different concentrations of 0, 5, 10, 25, and 50 M H 2 O 2 (prepared by diluting an aliquot of 30% (v/v) H 2 O 2 in 1 PBS) for 24 h, at 37C and shaking at 150 rpm