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The membrane was incubated with rabbit anti-GPX1 antigen affinity purified monoclonal antibody (Catalog # M01019-1) at 1:500 overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT

Animal studies suggest high tolerance and no significant toxicity, even at large doses [44]

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