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R&D Systems, Minneapolis, MN) in RPMI-1640 (Gibco, Detroit, MI) medium supplemented with 2% penicillin/streptomycin (Gibco), 20 mM HEPES (Gibco) and 5% pooled normal human heat-inactivated and sterile-filtered AB serum (Innovative Research, Novi, MI) for 7 days, with a media change on day 5 with fresh M-CSF (10 ng/mL)
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Those who took weekly semaglutide had a 20 percent lower risk of serious heart events like heart attacks and strokes compared to those who did not
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Nathan DM, Schreiber E, Fogel H, Mojsov S, Habener JF
