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A total volume of 30 l of each was added to a well of a 96-well plate
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J Mater Chem B (2014) 2(28):456471
Sorting vector construction and gene cloning The DNA sequences encoding the GST, the N-terminal signal peptides, and the C-terminal membrane-placing module of the six GPI/TMDs (Supplementary Table S1) were synthesized and supplied in pUC57a by GENEWIZ