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The process includes: Resin selection and preparation Amino acid coupling Deprotection steps Washing and filtration between steps Cleavage and Deprotection After the peptide chain is complete, it needs to be cleaved from the resin and deprotected

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After adding 1 ml of G-buffer, the sample was transferred to a 2-ml Eppendorf tube containing thrombin, to cleave off the streptavidin tag, and incubated at 4C while rotating overnight

JAMA 331:10071008 SELECT Trial Investigators (2023) Semaglutide and cardiovascular outcomes in obesity without diabetes

This condition exists in two main forms: non-alcoholic fatty liver disease (NAFLD) , which affects individuals who drink little or no alcohol, and alcohol-related liver disease (ARLD) , caused by excessive alcohol consumption

& Caucci, S